mac2 macrophage rat anti mouse mac2  (Cedarlane)

Journal:

Article Title: 4SC-101, A Novel Small Molecule Dihydroorotate Dehydrogenase Inhibitor, Suppresses Systemic Lupus Erythematosus in MRL-(Fas)lpr Mice

doi: 10.2353/ajpath.2010.091227

Figure Lengend Snippet: 4SC-101 improves renal histopathology in MRLlpr/lpr mice. A: Renal sections of 22-week-old MRLlpr/lpr mice were stained with periodic acid Schiff and antibodies for IgG and CD3 (T cells) as indicated. Images are representative for seven to 12 mice in each group (original magnification, ×100 [PAS], ×400 [IgG], ×100 [CD3]). B: Morphometry was performed on IgG and PAS stained sections to quantify glomerular IgG deposits (score ranging from 0 to 3), the lupus nephritis disease activity index (score ranging from 0 to 24), and the lupus nephritis chronicity index (score ranging from 0 to 12). C: CD3+ T cells and Mac2+ interstitial cells were counted in 15 high power fields from renal sections of MRLlpr/lpr mice from all groups. Mac2+ cells were also counted in 15 glomeruli from mice of all groups of MRLlpr/lpr mice from all groups. Data represent means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 versus vehicle control group; ND, not detected.

Article Snippet: 21 The following primary antibodies were used: rat anti-mouse Mac2 (macrophages, Cederlane, ON, Canada, 1:50); anti-mouse CD3 (1:100, clone 500A2, BD Pharmingen, Heidelberg, Germany); and anti-mouse IgG 1 (1:100, {"type":"entrez-nucleotide","attrs":{"text":"M32015","term_id":"198797","term_text":"M32015"}} M32015 , Caltag Laboratories, Burlingame, CA).

Techniques: Histopathology, Staining, Activity Assay